The SCC is in the process of ramping up activities in line with the HMS Coronavirus response https://hms.harvard.edu/coronavirus. We will be re-opening the week of 6/15/20. We will start with library preparation from encapsulations that occured pre-shutdown. We are making a queue of users who will need to run after HMS resumes operation and right now we can start booking runs after July 1st.  If you need to see us at the core please contact us by e-mail to arrange a time. HMS researchers will need to complete the Harvard Chrimson Health Attestation to enter the building. Researchs from area affiliates can present your health attestation from your home institution and your ID and should be allowed to come up to the core.

Self service 10x Chromium encapsulations will be limited in June & July.  Full service encapsulation will be available if you need self service access please contact core staff directly.

Announcements

SCC fees and rates were updated Jan 2020. The existing rates will hold until further notice.

CITE-seq, Cell Hashing, and Nuc-seq is compatible with both 10x and inDrops.

NovaSeq runs of inDrops require that you recieve UN-TRIMMED Fastq files. Adapter trimming seems to be causing some loss of valid reads.

10x Chromium Available  We offer 10x Single Cell RNA-Seq or scATAC-Seq either as self-service or staff-assisted. Users need to buy their own reagents and can sign up for time to run themselves once they have received training.  

Policy Regarding Storage of Backup Encapsulated Samples              The SCC policy is to store backup encapsulated cells for about 1 year after your run date. If you need your backup emulsion please come retrieve it from the SCC within a few months of your run. Exceptions may be made if needed for your project, but this needs to be arranged with the SCC.

inDrops Libraries

library structure